Gelatinised hyphae are best observed in a weak KOH solution with Congo Red, which stains the hyphae and provides contrast against any intervening gel.

Choose this state if: the hyphae of the pileipellis surface are separated from one another by a gelatinous matrix. The gel may arise from the breakdown of the hyphal wall (in which case the hyphae are very narrow) or be secreted by the hyphae. Where the pileus is glutinous, the pileipellis hyphae are always distinctly gelatinised.