The hypoderm is a distinct layer of the pileipellis, below the surface layer. The trama is the flesh of the pileus, below the surface layer or layers that make up the pileipellis.

Gelatinised hyphae are best observed in a weak KOH solution with Congo Red, which stains the hyphae and provides contrast against any intervening gel.

Choose this state if: the hyphae of the hypoderm or the pileus trama are separated from one another by a gelatinous matrix. The gel may arise from the breakdown of the hyphal wall (in which case the hyphae are very narrow) or be secreted by the hyphae. Choose this option also when there are more than two layers in the pileipellis, and one of the lower layers (such as the mediopellis) is gelatinised.